BBI1206 Microbiology

1.week

Morfology of microbes and groups of mikrobes.

Shape of bacteria. Organization of unicellulars.

2. week  

Cultivation and inhibition of bacteria

3.week

Most important bacteria groups

4. week

Light utilization

5.week

Metabolism of procariota and respiration.

6. week

Utilization of microbes in industry.

7. week

Virusies

8. week

STERILISATION AND DISINFECTION

Procedures of sterilisation

Procedures of disinfection

Determination of the microbiological efficacy of disinfectants

INTRODUCTION TO THE USE OF PRACTICAL LABORATORY MICROSCOPES

Bright-field light microscopy

Fluorescence microscopy

9. week

BASIC ALGOLOGICAL METHODS

CELL- AND GERM-COUNTING METHODS

Determination of cell counts with microscope

PCR-based cell counts

Determination of germ counts based on cultivation

10. week

CFU-counting techniques

The membrane filter technique

The end point dilution technique (MPN method)

STRAIN CULTURE AND CULTIVATION-BASED TECHNIQUES

Demonstration of microbes in the environment

Preparation of microbiological culture media

Basic bacterial cultivation techniques

Spread plate and pour plate methods

11. week

Pheno- and genotypic characterisation of bacterial strains

Colony- and cell morphology, staining procedures

Study of bacterial enzymes

SAMPLING METHODS IN MICROBIOLOGY ..

 Sampling from various environments

Collection of air samples

Determination of air microbs

Collection of soil samples

Determination of soil microbs

12. week

Sampling the surface of objects

Hygienic control of the hands of operators

Determination of surface microbs

Isolation and streak plate technique

Anaerobic cultivation techniques

Transfer, maintenance and storage of pure cultures

Investigation of cosmetic samples

Investigation of food samples

13. week

Investigation of FUNGI

14 week

Investigation of Virus

electronmicroscop

Requirement: Attending the practical course is obligatory.

Term grade:

1.Preparation of a laboratory report. Presentation and evaluation of the results of the experiments and tests carried out.                         

2.Writing tests. 

The average of the grade of points 1. and 2.